Journal of the International Aroid Society, Inc.
Volume 26, Number 1 (2003); Pages 120-124
Enhanced histological technique for observation of spathe pigmentation in Anthurium species and hybrids
By J. Ehrenberger and Adelheid R. Kuehnle     (Buy)


A total of 12 Anthurium species and hybrids were examined histologically for pigmentation in spathes using a new method employing vacuum infiltration of spathe tissue with polyethylene glycol (PEG) priorto cross-sectioning. PEG infiltration displaces intercellular air spaces between cells. This method greatly improved the clarity of the cross sections and consequently improved observations of spatial localization of anthocyanins and chloroplasts.


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