Pollen germination

From: Marc Gibernau gibernau at aurore.cict.fr> on 2002.06.10 at 15:35:37(8981) Dear Aroiders, I write a single message to answer to the different questions. Sorry for those who are not interested by the question. Apparently, nobody tried to test pollen viability by germiantions in vivo. I used the Brewbaker-Kwack Medium (10% sucrose, 100mg/L Boric acid, 300 mg/L Calcium nitrate, 200 mg/L Magnesium sulfate heptahydrate, 100 mg/L Potassium nitrate and distilled water. I only obtain 20-30% germination rates for Arum italicum. Apparently, there is a "better" medium for trinucleate pollen species called "Leduc, Monnier and Douglas" with a lot of new nutrients. I shall test it next year when the Arum will flower again!!! Sincerely yours, Marc Gibernau

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