titanum pollen fertility question

From: bonaventure at optonline.net (bonaventure at optonline.net) on 2008.06.04 at 16:23:27(17732) Would titanum pollen (thanks Fevzi) stored dry in a paper envelope in a kitchen refrigerator be viable after 2 months? I just dropped it into the spathe of my first outdoor blooming konjac. Bonaventure Magrys +More -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.gizmoworks.com/mailman/private/aroid-l/attachments/20080604/3faa29ed/attachment-0001.htm

From: bill.weaver at hp.com (Weaver, Bill) on 2008.06.04 at 21:32:28(17736) I would think there could be a problem if it is a standard 'frost free' freezer. These units stay 'frost free' by actually turning on an internal heater to melt off the frost and then re-freezing everything again. A possible way around this would be to put the pollen between two blocks of 'blue ice' so that the pollen itself is never allowed to defrost. Bill +More ________________________________ From: aroid-l-bounces at gizmoworks.com [mailto:aroid-l-bounces at gizmoworks.com] On Behalf Of bonaventure at optonline.net Sent: Wednesday, June 04, 2008 9:23 AM To: Discussion of aroids Subject: [Aroid-l] titanum pollen fertility question Would titanum pollen (thanks Fevzi) stored dry in a paper envelope in a kitchen refrigerator be viable after 2 months? I just dropped it into the spathe of my first outdoor blooming konjac. Bonaventure Magrys -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.gizmoworks.com/mailman/private/aroid-l/attachments/20080604/bd065bfd/attachment-0001.htm

From: Steve at ExoticRainforest.com (ExoticRainforest) on 2008.06.04 at 21:39:37(17738) Bonaventure, you might want to read what Julius has to say about this here: http://www.exoticrainforest.com/Natural%20and%20artificial%20pollination%20in%20aroids.html Steve Lucas +More www.ExoticRainforest.com Would titanum pollen (thanks Fevzi) stored dry in a paper envelope in a kitchen refrigerator be viable after 2 months? I just dropped it into the spathe of my first outdoor blooming konjac. Bonaventure Magrys -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.gizmoworks.com/mailman/private/aroid-l/attachments/20080604/996cae00/attachment-0001.htm

From: bonaventure at optonline.net (bonaventure at optonline.net) on 2008.06.06 at 18:52:21(17771) Thanks Steve, I was afraid to freeze it having no cryoprotectant, eg. glycerin, and it being titanum pollen and all. But it was dry, double wrapped in paper and in an envelope, let come up to room temperature slowly, and used that day. The konjac recipient was just starting to unfurl, so no chance of the female flowers being past receptivity or males shedding their own pollen afterwards. As a matter of fact, it only became odiferous the next day. There was almost half a teaspoonful of titanum pollen (thanks again Fevzi!) and this went "down the tube" all around the spadix as far down as I could pour it. This was followed by vigorous shaking and tapping of the base of the spathe for about a minute (oh God, I hope the neighbors weren't watching!). I hope that gravity and the flies have done the rest. I do believe that konjac is not big enough ;-) , and the introduction of titanum genes will greatly improve its size, introduce hybrid vigor (as if it needs that) and a percentage of seedlings will survive our zone 7 gardens here. On the other hand, one may get a more vigorous and rot resistant hardier titanum like Amorphophallus. Bonaventure +More -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.gizmoworks.com/mailman/private/aroid-l/attachments/20080606/028aaab2/attachment-0001.htm

From: Steve at ExoticRainforest.com (ExoticRainforest) on 2008.06.07 at 04:09:06(17777) I'm certainly not an expert, but I'm doing all I can to master this with the help of a bunch of great people. Sounds to me like you did it right. Obviously, some time will tell. Steve +More Thanks Steve, I was afraid to freeze it having no cryoprotectant, eg. glycerin, and it being titanum pollen and all. But it was dry, double wrapped in paper and in an envelope, let come up to room temperature slowly, and used that day. The konjac recipient was just starting to unfurl, so no chance of the female flowers being past receptivity or males shedding their own pollen afterwards. As a matter of fact, it only became odiferous the next day. There was almost half a teaspoonful of titanum pollen (thanks again Fevzi!) and this went "down the tube" all around the spadix as far down as I could pour it. This was followed by vigorous shaking and tapping of the base of the spathe for about a minute (oh God, I hope the neighbors weren't watching!). I hope that gravity and the flies have done the rest. I do believe that konjac is not big enough ;-) , and the introduction of titanum genes will greatly improve its size, introduce hybrid vigor (as if it needs that) and a percentage of seedlings will survive our zone 7 gardens here. On the other hand, one may get a more vigorous and rot resistant hardier titanum like Amorphophallus. Bonaventure -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.gizmoworks.com/mailman/private/aroid-l/attachments/20080606/f2512c00/attachment.htm

From: crogers at ecoanalysts.com (Christopher Rogers) on 2008.06.08 at 02:46:52(17799) I am really excited to see what you come up with . . . D. Christopher Rogers +More Senior Invertebrate Ecologist/ Taxonomist ((,///////////=======< EcoAnalysts, Inc. 1.530.756.4481 1.530. 383.4798 (cell) 1307 "L" Street Davis, CA 95616 USA ?Invertebrate Taxonomy ?Endangered Species ?Ecological Studies ?Bioassessment ?Invasive Species ?Plankton ?Phycology Moscow, Idaho ? Bozeman, Montana ? Davis, California ? Joplin, Missouri Selinsgrove, Pennsylvania ecoanalysts.com From: aroid-l-bounces at gizmoworks.com [mailto:aroid-l-bounces at gizmoworks.com] On Behalf Of bonaventure at optonline.net Sent: Friday, June 06, 2008 11:52 AM To: Discussion of aroids Subject: Re: [Aroid-l] titanum pollen fertility question Thanks Steve, I was afraid to freeze it having no cryoprotectant, eg. glycerin, and it being titanum pollen and all. But it was dry, double wrapped in paper and in an envelope, let come up to room temperature slowly, and used that day. The konjac recipient was just starting to unfurl, so no chance of the female flowers being past receptivity or males shedding their own pollen afterwards. As a matter of fact, it only became odiferous the next day. There was almost half a teaspoonful of titanum pollen (thanks again Fevzi!) and this went "down the tube" all around the spadix as far down as I could pour it. This was followed by vigorous shaking and tapping of the base of the spathe for about a minute (oh God, I hope the neighbors weren't watching!). I hope that gravity and the flies have done the rest. I do believe that konjac is not big enough ;-) , and the introduction of titanum genes will greatly improve its size, introduce hybrid vigor (as if it needs that) and a percentage of seedlings will survive our zone 7 gardens here. On the other hand, one may get a more vigorous and rot resistant hardier titanum like Amorphophallus. Bonaventure -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.gizmoworks.com/mailman/private/aroid-l/attachments/20080607/7521b1e0/attachment-0001.htm

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