Tissue Culture ? Can common folk do it?

From: santoury at aol.com on 2010.09.09 at 21:54:00(21426) I'm sure this has been answered before, but nothing on the internet shows any "common folk" doing tissue culture work to propagate their own plants. I'd love to do it, if possible. Granted, my facilities are not "scientific." But, boy, if I could grow plants from pieces of leaves - I'd sure love to. I'd love to hear about any experience you may have had with this endeavor. Thanks! Jude HTML +More ----------MB_8CD1E80A51306C4_1A54_7B2A_webmail-m088.sysops.aol.com-- --==============�00939076395537889=

From: "plantguy at zoominternet.net" <plantguy at zoominternet.net> on 2010.09.09 at 23:09:55(21428) Hi Jude, I must admit, I have often thought of trying it in my lab which is complete with anything that would be necessary, but have never found the time or actual inclination to have a stab at it. There are a lot of sites on the internet that show you how to do this as a home-TC person however. Sterility will be the biggest problem, but can be solved with 70% EtOH and a home-made hood.....also a alcohol burner. I've always thought the biggest issue would be which formulation to make for the agar you are doing the TC on as there are a million possible supplements that can be added (the agar also needs to be sterilized which is typically done in an autoclave). Best of luck if you give it a try and I would be interested in hearing your results. +More Dan Gibsonia, PA Original Message: ----------------- From: santoury@aol.com Date: Thu, 09 Sep 2010 17:54:00 -0400 To: aroid-l@www.gizmoworks.com, pbs@lists.ibiblio.org Subject: [Aroid-l] Tissue Culture ? Can common folk do it? I'm sure this has been answered before, but nothing on the internet shows any "common folk" doing tissue culture work to propagate their own plants. I'd love to do it, if possible. Granted, my facilities are not "scientific." But, boy, if I could grow plants from pieces of leaves - I'd sure love to. I'd love to hear about any experience you may have had with this endeavor. Thanks! Jude -------------------------------------------------------------------- mail2web.com - Microsoft� Exchange solutions from a leading provider - http://link.mail2web.com/Business/Exchange _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l

From: Don Martinson <llmen at wi.rr.com> on 2010.09.09 at 23:57:10(21430) In general, tissue culture is a very exacting and not inexpensive endeavor and not really intended for (no offense intended) us “common folk”. I’m not saying it can’t be done, but I don’t believe it is easily achieved without specialized materials and lots of expert help. Don Martinson HTML +More Milwaukee, Wisconsin Mailto:llmen@wi.rr.com On 9/9/10 4:54 PM, "santoury@aol.com" wrote: I'm sure this has been answered before, but nothing on the internet shows any "common folk" doing tissue culture work to propagate their own plants. I'd love to do it, if possible. Granted, my facilities are not "scientific." But, boy, if I could grow plants from pieces of leaves - I'd sure love to. I'd love to hear about any experience you may have had with this endeavor. Thanks! Jude _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l --B_3366903431_1702375-- --==============V84019717367033815=

From: "Sherry Gates" <TheTropix at msn.com> on 2010.09.10 at 00:33:13(21431) I excitedly 2nd this request! thanks, sherry ----- Original Message ----- HTML +More From: santoury@aol.com To: aroid-l@www.gizmoworks.com ; pbs@lists.ibiblio.org Sent: Thursday, September 09, 2010 4:54 PM Subject: [Aroid-l] Tissue Culture ? Can common folk do it? I'm sure this has been answered before, but nothing on the internet shows any "common folk" doing tissue culture work to propagate their own plants. I'd love to do it, if possible. Granted, my facilities are not "scientific." But, boy, if I could grow plants from pieces of leaves - I'd sure love to. I'd love to hear about any experience you may have had with this endeavor. Thanks! Jude _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l ------=_NextPart_000_024E_01CB5055.D833B260-- --==============771367435119934961=

From: "StroWi at t-online.de" <StroWi at t-online.de> on 2010.09.10 at 05:31:01(21433) Jude, there is a yahoo group on hometissueculture... http://tech.groups.yahoo.com/group/hometissueculture/ +More Happy growing, Bernhard. -----Original Message----- > Date: Thu, 09 Sep 2010 23:54:00 +0200 > Subject: [Aroid-l] Tissue Culture ? Can common folk do it? > From: santoury@aol.com > To: aroid-l@www.gizmoworks.com, pbs@lists.ibiblio.org > I'm sure this has been answered before, but nothing on the internet shows any "common folk" doing tissue culture work to propagate their own plants. I'd love to do it, if possible. Granted, my facilities are not "scientific." But, boy, if I could grow plants from pieces of leaves - I'd sure love to. I'd love to hear about any experience you may have had with this endeavor. Thanks! Jude _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l

From: Zach DuFran <zdufran at wdtinc.com> on 2010.09.10 at 13:55:21(21435) Jude- One of our IAS members (Ghazanfar Ghori) has a very nice blog where he documents his work with Cryptocorynes. He does a lot of tissue culture. Check it out: http://kryptokoryne.aquaticscape.com/ Zach HTML +More From: aroid-l-bounces@www.gizmoworks.com [mailto:aroid-l-bounces@www.gizmoworks.com] On Behalf Of santoury@aol.com Sent: Thursday, September 09, 2010 4:54 PM To: aroid-l@www.gizmoworks.com; pbs@lists.ibiblio.org Subject: [Aroid-l] Tissue Culture ? Can common folk do it? I'm sure this has been answered before, but nothing on the internet shows any "common folk" doing tissue culture work to propagate their own plants. I'd love to do it, if possible. Granted, my facilities are not "scientific." But, boy, if I could grow plants from pieces of leaves - I'd sure love to. I'd love to hear about any experience you may have had with this endeavor. Thanks! Jude --_000_792F4A6158EAE44189316A2EAD8CCBB91D675Drainwdtinccom_-- --==============e27905302172083030=

From: Steve Marak <samarak at gizmoworks.com> on 2010.09.11 at 17:29:35(21443) My wife's setup involves a pressure cooker, an electronic kitchen balance that reads in grams, and a "hood" made by buying a large clear plastic bin at Wal-Mart, turning it upside down, and cutting a hole in the side and taping a clear plastic flap over it. After trying several options, she's now using regular home canning jars (with the plastic lids, not the metal ones) to hold the medium. We happened to already have the pressure cooker and electronic kitchen balance, but other than those, the most expensive thing has been purchasing the media, which Cathy gets from PhytoTech ( http://www.phytotechlab.com ). She's buying pre-mixed formulations they offer, which require only the addition of sugar. At the moment she's focused on learning to flask orchid seed efficiently, rather than tissue culture, but she has done a little TC in that kind of setup when she was teaching high school, so it can be done. Carolina Biological ( http://www.carolina.com ) offers TC "kits" designed for teachers/students/home enthusiasts (put "tissue culture" in the search box). They won't set you up to do TC of your plants at home, but can give you a feel for the kind of lab techniques you need (and help give you the confidence that you can do it), and several of them are pretty cheap - the sundew TC kit is less than $25 US. Sterilization of the environment in the hood is key, of course. Some people use a spray bottle of ethanol, but not only is it very "fumey", it's very flammable, so Cathy uses other options. You can use dilute bleach, or some people prefer a calcium hypochlorite (household bleach is a sodium hypochlorite solution) solution instead. Aaron Hicks, at his Orchid Seedbank Project, has lots of great advice from his experience flasking orchids which would apply in TC also. Check out http://members.cox.net/ahicks51/osp/ Steve +More On Thu, 9 Sep 2010, Sherry Gates wrote: > I excitedly 2nd this request! > thanks, sherry > ----- Original Message ----- > From: santoury@aol.com > Subject: [Aroid-l] Tissue Culture ? Can common folk do it? > > I'm sure this has been answered before, but nothing on the internet > shows any "common folk" doing tissue culture work to propagate their > own plants. I'd love to do it, if possible. Granted, my facilities are > not "scientific." But, boy, if I could grow plants from pieces of > leaves - I'd sure love to. I'd love to hear about any experience you > may have had with this endeavor. Thanks! Jude -- Steve Marak -- samarak@gizmoworks.com _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l

From: santoury at aol.com on 2010.09.11 at 21:01:20(21445) Hey there Thank you very much - Here I was thinking, hoping, that it would just be a matter of cutting up some leaves, and putting them on agar... The whole spraying, chemicals and such - no thanks! Darn. One could only hope, right? :) HTML +More -----Original Message----- From: Steve Marak To: Discussion of aroids Sent: Sat, Sep 11, 2010 1:29 pm Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it? My wife's setup involves a pressure cooker, an electronic kitchen balance that reads in grams, and a "hood" made by buying a large clear plastic bin at Wal-Mart, turning it upside down, and cutting a hole in the side and taping a clear plastic flap over it. After trying several options, she's now using regular home canning jars (with the plastic lids, not the metal ones) to hold the medium. We happened to already have the pressure cooker and electronic kitchen balance, but other than those, the most expensive thing has been purchasing the media, which Cathy gets from PhytoTech ( http://www.phytotechlab.com ). She's buying pre-mixed formulations they offer, which require only the addition of sugar. At the moment she's focused on learning to flask orchid seed efficiently, rather than tissue culture, but she has done a little TC in that kind of setup when she was teaching high school, so it can be done. Carolina Biological ( http://www.carolina.com ) offers TC "kits" designed for teachers/students/home enthusiasts (put "tissue culture" in the search box). They won't set you up to do TC of your plants at home, but can give you a feel for the kind of lab techniques you need (and help give you the confidence that you can do it), and several of them are pretty cheap - the sundew TC kit is less than $25 US. Sterilization of the environment in the hood is key, of course. Some people use a spray bottle of ethanol, but not only is it very "fumey", it's very flammable, so Cathy uses other options. You can use dilute bleach, or some people prefer a calcium hypochlorite (household bleach is a sodium hypochlorite solution) solution instead. Aaron Hicks, at his Orchid Seedbank Project, has lots of great advice from his experience flasking orchids which would apply in TC also. Check out http://members.cox.net/ahicks51/osp/ Steve On Thu, 9 Sep 2010, Sherry Gates wrote: > I excitedly 2nd this request! > thanks, sherry > ----- Original Message ----- > From: santoury@aol.com > Subject: [Aroid-l] Tissue Culture ? Can common folk do it? > > I'm sure this has been answered before, but nothing on the internet > shows any "common folk" doing tissue culture work to propagate their > own plants. I'd love to do it, if possible. Granted, my facilities are > not "scientific." But, boy, if I could grow plants from pieces of > leaves - I'd sure love to. I'd love to hear about any experience you > may have had with this endeavor. Thanks! Jude -- Steve Marak -- samarak@gizmoworks.com _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l ----------MB_8CD200B9EBE6E87_11C0_10E30_webmail-m002.sysops.aol.com-- --==============29508571198032742=

From: "Steve Hatfield" <moondogman at comcast.net> on 2010.09.11 at 21:11:37(21446) http://www.hometissueculture.org/ I found this a few months ago.It looks doable on a budget. I think a tinkerer could come up with most of the things in the kits. Check out these videos on YouTube Micropropagation Part I Intro. http://www.youtube.com/watch?v=KYvJByYrSPg looks doable. Steve +More -----Original Message----- From: aroid-l-bounces@www.gizmoworks.com [mailto:aroid-l-bounces@www.gizmoworks.com] On Behalf Of StroWi@t-online.de Sent: Friday, September 10, 2010 1:31 AM To: Discussion of aroids Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it? Jude, there is a yahoo group on hometissueculture... http://tech.groups.yahoo.com/group/hometissueculture/ Happy growing, Bernhard. -----Original Message----- > Date: Thu, 09 Sep 2010 23:54:00 +0200 > Subject: [Aroid-l] Tissue Culture ? Can common folk do it? > From: santoury@aol.com > To: aroid-l@www.gizmoworks.com, pbs@lists.ibiblio.org > I'm sure this has been answered before, but nothing on the internet shows any "common folk" doing tissue culture work to propagate their own plants. I'd love to do it, if possible. Granted, my facilities are not "scientific." But, boy, if I could grow plants from pieces of leaves - I'd sure love to. I'd love to hear about any experience you may have had with this endeavor. Thanks! Jude _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l

From: "plantguy at zoominternet.net" <plantguy at zoominternet.net> on 2010.09.11 at 21:12:56(21447) Hi Steve, Sounds like your wife is having some fun :o) I guess I forgot about a nice place to look up info and get a free book on the subject. If you go to www.sigma-aldrich.com you can find a huge number of things by putting plant tissue culture in the search box. The only problem is that you can get overwhelmed by the sheer volume of info.....beware if you really want scientific info.....there is a massive amount of it. +More I actually got this free manual quite awhile ago when I was contemplating doing this, but as usual, life got in the way. Here is a link....scroll down and ask for the catalogue....do not know if you have to be in academia to get it.......I will request one to find out: http://www.sigmaaldrich.com/life-science/molecular-biology/plant-biotechnolo gy/learning-center/tissue-culture-guide.html Needless to say, unless you just want to have fun, you need to have a plant rare enough to warrant TC. Dan Gibsonia, PA Original Message: ----------------- From: Steve Marak samarak@gizmoworks.com Date: Sat, 11 Sep 2010 12:29:35 -0500 (CDT) To: aroid-l@www.gizmoworks.com Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it? My wife's setup involves a pressure cooker, an electronic kitchen balance that reads in grams, and a "hood" made by buying a large clear plastic bin at Wal-Mart, turning it upside down, and cutting a hole in the side and taping a clear plastic flap over it. After trying several options, she's now using regular home canning jars (with the plastic lids, not the metal ones) to hold the medium. We happened to already have the pressure cooker and electronic kitchen balance, but other than those, the most expensive thing has been purchasing the media, which Cathy gets from PhytoTech ( http://www.phytotechlab.com ). She's buying pre-mixed formulations they offer, which require only the addition of sugar. At the moment she's focused on learning to flask orchid seed efficiently, rather than tissue culture, but she has done a little TC in that kind of setup when she was teaching high school, so it can be done. Carolina Biological ( http://www.carolina.com ) offers TC "kits" designed for teachers/students/home enthusiasts (put "tissue culture" in the search box). They won't set you up to do TC of your plants at home, but can give you a feel for the kind of lab techniques you need (and help give you the confidence that you can do it), and several of them are pretty cheap - the sundew TC kit is less than $25 US. Sterilization of the environment in the hood is key, of course. Some people use a spray bottle of ethanol, but not only is it very "fumey", it's very flammable, so Cathy uses other options. You can use dilute bleach, or some people prefer a calcium hypochlorite (household bleach is a sodium hypochlorite solution) solution instead. Aaron Hicks, at his Orchid Seedbank Project, has lots of great advice from his experience flasking orchids which would apply in TC also. Check out http://members.cox.net/ahicks51/osp/ Steve On Thu, 9 Sep 2010, Sherry Gates wrote: > I excitedly 2nd this request! > thanks, sherry > ----- Original Message ----- > From: santoury@aol.com > Subject: [Aroid-l] Tissue Culture ? Can common folk do it? > > I'm sure this has been answered before, but nothing on the internet > shows any "common folk" doing tissue culture work to propagate their > own plants. I'd love to do it, if possible. Granted, my facilities are > not "scientific." But, boy, if I could grow plants from pieces of > leaves - I'd sure love to. I'd love to hear about any experience you > may have had with this endeavor. Thanks! Jude -- Steve Marak -- samarak@gizmoworks.com _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l -------------------------------------------------------------------- mail2web - Check your email from the web at http://link.mail2web.com/mail2web _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l

From: "Nancy Greig" <ngreig at hmns.org> on 2010.09.12 at 14:26:26(21453) I seem to recall that Steve Lucas mentioned to me that Tony Avent had written an article for the IAS journal on growing Amorphophallus plants from leaf cuttings. Does anyone know the issue number? Nancy Greig +More Director, Cockrell Butterfly Center Houston Museum of Natural Science One Hermann Circle Drive Houston, TX 77030-1799 tel 713-639-4742 fax 713-639-4788 www.hmns.org -----Original Message----- From: aroid-l-bounces@www.gizmoworks.com on behalf of santoury@aol.com Sent: Sat 9/11/2010 4:01 PM To: aroid-l@www.gizmoworks.com Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it? Hey there Thank you very much - Here I was thinking, hoping, that it would just be a matter of cutting up some leaves, and putting them on agar... The whole spraying, chemicals and such - no thanks! Darn. One could only hope, right? :) -----Original Message----- From: Steve Marak To: Discussion of aroids Sent: Sat, Sep 11, 2010 1:29 pm Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it? My wife's setup involves a pressure cooker, an electronic kitchen balance that reads in grams, and a "hood" made by buying a large clear plastic bin at Wal-Mart, turning it upside down, and cutting a hole in the side and taping a clear plastic flap over it. After trying several options, she's now using regular home canning jars (with the plastic lids, not the metal ones) to hold the medium. We happened to already have the pressure cooker and electronic kitchen balance, but other than those, the most expensive thing has been purchasing the media, which Cathy gets from PhytoTech ( http://www.phytotechlab.com ). She's buying pre-mixed formulations they offer, which require only the addition of sugar. At the moment she's focused on learning to flask orchid seed efficiently, rather than tissue culture, but she has done a little TC in that kind of setup when she was teaching high school, so it can be done. Carolina Biological ( http://www.carolina.com ) offers TC "kits" designed for teachers/students/home enthusiasts (put "tissue culture" in the search box). They won't set you up to do TC of your plants at home, but can give you a feel for the kind of lab techniques you need (and help give you the confidence that you can do it), and several of them are pretty cheap - the sundew TC kit is less than $25 US. Sterilization of the environment in the hood is key, of course. Some people use a spray bottle of ethanol, but not only is it very "fumey", it's very flammable, so Cathy uses other options. You can use dilute bleach, or some people prefer a calcium hypochlorite (household bleach is a sodium hypochlorite solution) solution instead. Aaron Hicks, at his Orchid Seedbank Project, has lots of great advice from his experience flasking orchids which would apply in TC also. Check out http://members.cox.net/ahicks51/osp/ Steve On Thu, 9 Sep 2010, Sherry Gates wrote: > I excitedly 2nd this request! > thanks, sherry > ----- Original Message ----- > From: santoury@aol.com > Subject: [Aroid-l] Tissue Culture ? Can common folk do it? > > I'm sure this has been answered before, but nothing on the internet > shows any "common folk" doing tissue culture work to propagate their > own plants. I'd love to do it, if possible. Granted, my facilities are > not "scientific." But, boy, if I could grow plants from pieces of > leaves - I'd sure love to. I'd love to hear about any experience you > may have had with this endeavor. Thanks! Jude -- Steve Marak -- samarak@gizmoworks.com _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l ------_=_NextPart_001_01CB5286.B8BFFDBE _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l ------_=_NextPart_001_01CB5286.B8BFFDBE--

From: ExoticRainforest <Steve at ExoticRainforest.com> on 2010.09.12 at 21:42:38(21454) Nancy, it is in volume 30. Steve HTML +More www.ExoticRainforest.com On 9/12/2010 09:26, Nancy Greig wrote: I seem to recall that Steve Lucas mentioned to me that Tony Avent had written an article for the IAS journal on growing Amorphophallus plants from leaf cuttings. Does anyone know the issue number? Nancy Greig Director, Cockrell Butterfly Center Houston Museum of Natural Science One Hermann Circle Drive Houston, TX 77030-1799 tel 713-639-4742 fax 713-639-4788 www.hmns.org -----Original Message----- From: aroid-l-bounces@www.gizmoworks.com on behalf of santoury@aol.com Sent: Sat 9/11/2010 4:01 PM To: aroid-l@www.gizmoworks.com Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it? Hey there Thank you very much - Here I was thinking, hoping, that it would just be a matter of cutting up some leaves, and putting them on agar... The whole spraying, chemicals and such - no thanks! Darn. One could only hope, right? :) -----Original Message----- From: Steve Marak To: Discussion of aroids Sent: Sat, Sep 11, 2010 1:29 pm Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it? My wife's setup involves a pressure cooker, an electronic kitchen balance that reads in grams, and a "hood" made by buying a large clear plastic bin at Wal-Mart, turning it upside down, and cutting a hole in the side and taping a clear plastic flap over it. After trying several options, she's now using regular home canning jars (with the plastic lids, not the metal ones) to hold the medium. We happened to already have the pressure cooker and electronic kitchen balance, but other than those, the most expensive thing has been purchasing the media, which Cathy gets from PhytoTech ( http://www.phytotechlab.com ). She's buying pre-mixed formulations they offer, which require only the addition of sugar. At the moment she's focused on learning to flask orchid seed efficiently, rather than tissue culture, but she has done a little TC in that kind of setup when she was teaching high school, so it can be done. Carolina Biological ( http://www.carolina.com ) offers TC "kits" designed for teachers/students/home enthusiasts (put "tissue culture" in the search box). They won't set you up to do TC of your plants at home, but can give you a feel for the kind of lab techniques you need (and help give you the confidence that you can do it), and several of them are pretty cheap - the sundew TC kit is less than $25 US. Sterilization of the environment in the hood is key, of course. Some people use a spray bottle of ethanol, but not only is it very "fumey", it's very flammable, so Cathy uses other options. You can use dilute bleach, or some people prefer a calcium hypochlorite (household bleach is a sodium hypochlorite solution) solution instead. Aaron Hicks, at his Orchid Seedbank Project, has lots of great advice from his experience flasking orchids which would apply in TC also. Check out http://members.cox.net/ahicks51/osp/ Steve On Thu, 9 Sep 2010, Sherry Gates wrote: I excitedly 2nd this request! thanks, sherry ----- Original Message ----- From: santoury@aol.com Subject: [Aroid-l] Tissue Culture ? Can common folk do it? I'm sure this has been answered before, but nothing on the internet shows any "common folk" doing tissue culture work to propagate their own plants. I'd love to do it, if possible. Granted, my facilities are not "scientific." But, boy, if I could grow plants from pieces of leaves - I'd sure love to. I'd love to hear about any experience you may have had with this endeavor. Thanks! Jude -- Steve Marak -- samarak@gizmoworks.com _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l --------------090500030504080901070804

From: "Derek Burch" <derek at horticulturist.com> on 2010.09.13 at 02:16:51(21457) Nancy, There is a searchable index on Aroid-l (saved me going to the files). 2007 30 124-138 Tony Avent Propagation of Amorphophallus by leaf petiole cuttings (Buy) He takes away a lot of the mystery. I haven't looked back in the Aroid-l archives, but this topic or one like it has come up in the past. It is worth getting to know all the things on aroid-l: Aroideana stuff, aroiders, cultivar registration, and many more gems, not to mention great galleries of images. It is not limited to members of IAS, although we all hope that it generates membership. Derek +More -----Original Message----- From: aroid-l-bounces@www.gizmoworks.com [mailto:aroid-l-bounces@www.gizmoworks.com] On Behalf Of Nancy Greig Sent: Sunday, September 12, 2010 10:26 AM To: Discussion of aroids Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it? I seem to recall that Steve Lucas mentioned to me that Tony Avent had written an article for the IAS journal on growing Amorphophallus plants from leaf cuttings. Does anyone know the issue number? Nancy Greig Director, Cockrell Butterfly Center Houston Museum of Natural Science One Hermann Circle Drive Houston, TX 77030-1799 tel 713-639-4742 fax 713-639-4788 www.hmns.org -----Original Message----- From: aroid-l-bounces@www.gizmoworks.com on behalf of santoury@aol.com Sent: Sat 9/11/2010 4:01 PM To: aroid-l@www.gizmoworks.com Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it? Hey there Thank you very much - Here I was thinking, hoping, that it would just be a matter of cutting up some leaves, and putting them on agar... The whole spraying, chemicals and such - no thanks! Darn. One could only hope, right? :) -----Original Message----- From: Steve Marak To: Discussion of aroids Sent: Sat, Sep 11, 2010 1:29 pm Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it? My wife's setup involves a pressure cooker, an electronic kitchen balance that reads in grams, and a "hood" made by buying a large clear plastic bin at Wal-Mart, turning it upside down, and cutting a hole in the side and taping a clear plastic flap over it. After trying several options, she's now using regular home canning jars (with the plastic lids, not the metal ones) to hold the medium. We happened to already have the pressure cooker and electronic kitchen balance, but other than those, the most expensive thing has been purchasing the media, which Cathy gets from PhytoTech ( http://www.phytotechlab.com ). She's buying pre-mixed formulations they offer, which require only the addition of sugar. At the moment she's focused on learning to flask orchid seed efficiently, rather than tissue culture, but she has done a little TC in that kind of setup when she was teaching high school, so it can be done. Carolina Biological ( http://www.carolina.com ) offers TC "kits" designed for teachers/students/home enthusiasts (put "tissue culture" in the search box). They won't set you up to do TC of your plants at home, but can give you a feel for the kind of lab techniques you need (and help give you the confidence that you can do it), and several of them are pretty cheap - the sundew TC kit is less than $25 US. Sterilization of the environment in the hood is key, of course. Some people use a spray bottle of ethanol, but not only is it very "fumey", it's very flammable, so Cathy uses other options. You can use dilute bleach, or some people prefer a calcium hypochlorite (household bleach is a sodium hypochlorite solution) solution instead. Aaron Hicks, at his Orchid Seedbank Project, has lots of great advice from his experience flasking orchids which would apply in TC also. Check out http://members.cox.net/ahicks51/osp/ Steve On Thu, 9 Sep 2010, Sherry Gates wrote: > I excitedly 2nd this request! > thanks, sherry > ----- Original Message ----- > From: santoury@aol.com > Subject: [Aroid-l] Tissue Culture ? Can common folk do it? > > I'm sure this has been answered before, but nothing on the internet > shows any "common folk" doing tissue culture work to propagate their > own plants. I'd love to do it, if possible. Granted, my facilities are > not "scientific." But, boy, if I could grow plants from pieces of > leaves - I'd sure love to. I'd love to hear about any experience you > may have had with this endeavor. Thanks! Jude -- Steve Marak -- samarak@gizmoworks.com _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l ------=_NextPart_000_0012_01CB52C8.339990B0 _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l ------=_NextPart_000_0012_01CB52C8.339990B0--

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