Department of Biology
University of Washington
Box 351800
Seattle, WA 98195-1800
On Thu, 18 Sep 2003, WEAVER,BILL (HP-USA,ex1) wrote:
> What was the timeframe before the new shoots appeared? I had to dig them up
> and repot them before anything happened.
>
> Bill
>
> -----Original Message-----
> From: Douglas Ewing [mailto:dewing@u.washington.edu]
> Sent: Thursday, September 18, 2003 8:36 AM
> To: 'aroid-l posting'
> Subject: Re: [aroid-l] A. Titanum cloned
>
>
> I too have been cloning titanum from leaf cuttings. We differ from Bill's
> procedure slightly:
>
> I use a 12" portion of the leaf with the mid-vein. I have not
> experimented with smaller cuttings.
>
> We apply no hormone. ( this is something I have avoided with all
> leaf cuttings in the past, as I was under the impression that auxin would
> promote root formation, but that it inhibits formation of shoots. Since we
> want both to happen with a leaf cutting, we do not apply. Tubers are
> shoots, perhaps this explains the difference in the gel vs. powder
> results?)
>
> We stick the cutting into a 8cm cube of rockwool, water, and
> encapsulate the entire affair in a terrarium made from 2 2liter
> pop-bottles. This creates almost total humidity, and cutting do not wilt.
> If/when fungus appears on the cutting, we dust with cinnamon. Tuber
> formation
> takes several months, then we wean from humidity by utilizing a pop-bottle
> with the bottom removed and the cap off, this allows a bit of air exchange
> for a few days , then the cutting is removed from the structure, potted up
> and placed on the open bench. Typically the orig. leaf tissue dies,
> followed by new leaf emergence. We also see multiple leaf emergence, which
> I am assuming will result in multiple tubers.
>
> I have not tried other sp. of this genus, but the fact that
> several species produce tubercles on the leaf naturally,( A. bulbifer)
> makes me think that all sp. might respond to this treatment.
>
>
> Doug
> _________________/\/\/\______________________
>
> Doug Ewing, Greenhouse Manager (206) 543-0436
> Department of Biology
> University of Washington
> Box 351800
> Seattle, WA 98195-1800
>
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