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  Re: [aroid-l] Hybrids and doubling chromosomes
From: plantguy at zoominternet.net> on 2003.11.22 at 00:50:32(10848)
Hi Brian,

Sounds like an interesting project. We use colchicine all the time to
disrupt microtubules and look at their role in vesicular trafficking of ion
channels in my lab, but I never before looked into it in terms of plants.
After reading your post and then a few articles I guess I'd say what the
heck, it's so easy why not give it a whirl. Obviously, all you'd need is
enough seed/plant material to try many concentrations and various time
points. I would caution that if you don't know what your doing with toxins
like this it wouldn't be a wise choice. Hopefully you have a good
analytical balance and a hood for working with these types of compounds. If
you're planning on using DMSO as a carrier I'd hope you would double
glove....better safe than sorry. Perhaps this would be a fun project for
next spring in the lab. It will be interesting to see what others familiar
with plant cell biology have to say!

Dan Devor

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