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Re: [aroid-l] Hybrids and doubling chromosomes
From: plantguy at zoominternet.net> on 2003.11.22 at 00:50:32(10848)|
Sounds like an interesting project. We use colchicine all the time to
disrupt microtubules and look at their role in vesicular trafficking of ion
channels in my lab, but I never before looked into it in terms of plants.
After reading your post and then a few articles I guess I'd say what the
heck, it's so easy why not give it a whirl. Obviously, all you'd need is
enough seed/plant material to try many concentrations and various time
points. I would caution that if you don't know what your doing with toxins
like this it wouldn't be a wise choice. Hopefully you have a good
analytical balance and a hood for working with these types of compounds. If
you're planning on using DMSO as a carrier I'd hope you would double
glove....better safe than sorry. Perhaps this would be a fun project for
next spring in the lab. It will be interesting to see what others familiar
with plant cell biology have to say!
----- Original Message -----|
Sent: Friday, November 21, 2003 6:23 PM
Subject: [aroid-l] Hybrids and doubling chromosomes
> I am interested in hearing if anyone has been doing hybrid work with any
> Aroids. Extremly interested in the storing of pollen in freezers or
> other ways. Would be interested in exact temps time periods and results.
> I have also heard of Hybrid amorphophallus though never seen a pic.
> Does this thing exist and if so why is their no info or pics of it. I
> understand the botanist point of view but from a collectors its very
> Last but not least has anyone tried chromosome doubling? Using chemicals
> on aroids like Colchincine? I have been reading up on it. It seems very
> interesting. Has anyone ever tried or working with this or other types
> of chemicals to produce tetraploids?
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