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  Re: [Aroid-l] 'Indian Giant' ploidylevel [was:Re: Sauromatum x
From: Christopher Rogers <branchiopod at gmail.com> on 2011.01.24 at 13:18:29
Nice!
=A0
You going to publish this in Aroidiana??
=A0
Happy days,
Christopher

On Fri, Jan 21, 2011 at 8:06 AM, StroWi@t-online.de <StroWi@t-online.de> wrote:

-----Original Message-----
> Date: Wed, 07 Jul 2010 21:03:55 +0200
> Subject: Re: [Aroid-l] Sauromatum x Pinellia hybrid.... and others...
> From: James Waddick <jwaddick@kc.rr.com>
> To: Discussion of aroids aroid-l@www.gizmoworks.com
....

> In this same discussion, the cv "Indian Giant' is brought to
> mind. This is a much larger form of S. venosum. Could it be a
> tetraploid? Has anyone compared chromosome numbers to the typical,
> smaller form? And is this, by the way, any more or less hardier than
> the typical form?



Dear Jim W.,

Aroiders,


already more than one year ago I compared the ploidy level of a normal form of Typhonium venosum (a genotype/tuber that came from Laos) with my=A0'Indian Giant' clone that was originally introduced by Tony Avent (Plant Delights Nursery). Furthermore I have the clone that was given to me as T. nubicum. but is probably identical with 'Indian Giant'.


For pictures see: (scroll down to the post from 06..11.2009)

http://www.amorphophallus-forum.de/viewtopic.php?f=3D9&t=3D316&start=3D30


I did not count the chromosomes but used flowcytometry to compare the DNA content.

In short words with=A0this method the DNA content of nuclei is measured after the DNA was=A0stained.

The measured values are shown in a graph.

The first peak represents the cells in the G1-phase; this would give the DNA-content of somatic cells. The second peak represents the nucelar DNA content of cell in the G2-phase after the replication of the DNA before the cell devides.


The graphs show the measurements of T. venosum 'Laos', T.venosum 'Indian Giant' and T. nubicum, respectively.


[img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_v_Laos.jpg[/img]

[img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_v_IndianGiant.jpg[/img]

[img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_nubicum.jpg[/img]


The results strongly indicate that the three clones do not differ in DNA content and therefore have the same ploidy level.

However, I did no chomosome counts, but if 'Indian Giant' would be a tetraploid of the normal form of T. venosum, the graph should show=A0a first peak at channel 100 for the G1-nuclei and a second at channel 200 for the G2-nulei. This is not the case; all three clones have the peaks=A0roughly at channel 50 and channel 100.



Best, Bernhard.


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--
D. Christopher Rogers
((,///////////=3D=3D=3D=3D=3D=3D=3D<
785.864.1714
Crustacean Taxonomist and Ecologist
Kansas Biological Survey
Kansas University, Higuchi Hall
2101 Constant Avenue, Lawrence, KS 66047-3759 USA

Associate Editor, Journal of Crustacean Biology

Vice President, Southwest Association of Freshwater Invertebrate Taxonomists SAFIT.ORG

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