-----Original Message-----
> Date: Wed, 07 Jul 2010 21:03:55 +0200
> Subject: Re: [Aroid-l] Sauromatum x Pinellia hybrid.... and others...
> From: James Waddick <jwaddick@kc.rr.com>
> To: Discussion of aroids aroid-l@www.gizmoworks.com
....
> In this same discussion, the cv "Indian Giant' is brought to
> mind. This is a much larger form of S. venosum. Could it be a
> tetraploid? Has anyone compared chromosome numbers to the typical,
> smaller form? And is this, by the way, any more or less hardier than
> the typical form?
Dear Jim W.,
Aroiders,
already more than one year ago I compared the ploidy level of a normal form of Typhonium venosum (a genotype/tuber that came from Laos) with my=A0'Indian Giant' clone that was originally introduced by Tony Avent (Plant Delights Nursery). Furthermore I have the clone that was given to me as T. nubicum. but is probably identical with 'Indian Giant'.
For pictures see: (scroll down to the post from 06..11.2009)
http://www.amorphophallus-forum.de/viewtopic.php?f=3D9&t=3D316&start=3D30
I did not count the chromosomes but used flowcytometry to compare the DNA content.
In short words with=A0this method the DNA content of nuclei is measured after the DNA was=A0stained.
The measured values are shown in a graph.
The first peak represents the cells in the G1-phase; this would give the DNA-content of somatic cells. The second peak represents the nucelar DNA content of cell in the G2-phase after the replication of the DNA before the cell devides.
The graphs show the measurements of T. venosum 'Laos', T.venosum 'Indian Giant' and T. nubicum, respectively.
[img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_v_Laos.jpg[/img]
[img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_v_IndianGiant.jpg[/img]
[img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_nubicum.jpg[/img]
The results strongly indicate that the three clones do not differ in DNA content and therefore have the same ploidy level.
However, I did no chomosome counts, but if 'Indian Giant' would be a tetraploid of the normal form of T. venosum, the graph should show=A0a first peak at channel 100 for the G1-nuclei and a second at channel 200 for the G2-nulei. This is not the case; all three clones have the peaks=A0roughly at channel 50 and channel 100.
Best, Bernhard.
IAS on Facebook
IAS on Instagram
