Re: [Aroid-l] Amorphophallus pollen request

From: Corey W <cewickliffe at gmail.com> on 2015.08.17 at 20:59:09 Very interesting to note, given that there are groups of pollen and seeds who have the "if it dries it dies" issue. Things like these are important to note for various species since in some cases dried pollen may perform better, and also how to store "humid" pollen (or seeds!) for long periods. It never ceases to amaze me that what won't work at "regular" freezer temperatures (due to ice crystals forming and fracturing cells maybe??) may work at -80. -80 is also significantly cheaper (and safer) to work with than liquid nitrogen! Kinda makes me wonder if that would work with the seeds too? Were the pollen samples ever tested for (length of) viability at other temperatures? Forgive me if there are good references about this already, my attempts to look into the subject found little to no information on tropical species that can't be dried other than "use fresh". Best, Corey On Fri, Aug 14, 2015 at 7:07 PM, Weaver, Bill (NorCal DCIS Team Lead) <bill.weaver@hp.com> wrote: And stranger still, it was not dried first. It was frozen 'fresh' as I was told for titanium pollen, "if it dries it dies". -----Original Message----- From: aroid-l-bounces@www.gizmoworks.com [mailto:aroid-l-bounces@www.gizmoworks.com] On Behalf Of Tom Croat Sent: Friday, August 14, 2015 3:38 PM To: 'Discussion of aroids' Subject: Re: [Aroid-l] Amorphophallus pollen request Dear Bill: Thank you so much for your good advice. I am astonished that it will last so long. I would think such temperatures would cause it to break to pieces. Tom -----Original Message----- From: aroid-l-bounces@www.gizmoworks.com [mailto:aroid-l-bounces@www.gizmoworks.com] On Behalf Of Weaver, Bill (NorCal DCIS Team Lead) Sent: Friday, August 14, 2015 3:24 PM To: Discussion of aroids; aroid-l@gizmoworks.com Cc: 'btankers@chicagobotanic.org' Subject: Re: [Aroid-l] Amorphophallus pollen request Pretty straight forward. At the end of the first day install foil trays above the female flowers and below the male flowers. (see attached) overnight the male flowers will produce pollen that can then be swept into the trays and the trays removed at the end of the day. The pollen should then be packaged and frozen and kept as cold as it can be. The pollen from the 2005 blooming of Trudy at UC Berkeley was kept in a DNA freezer at 80 degrees F below zero and was fully viable two years later when applied to Titania in 2007. -----Original Message----- From: aroid-l-bounces@www.gizmoworks.com [mailto:aroid-l-bounces@www.gizmoworks.com] On Behalf Of Tom Croat Sent: Thursday, August 13, 2015 4:39 PM To: aroid-l@gizmoworks.com Cc: 'btankers@chicagobotanic.org' Subject: [Aroid-l] FW: Amorphophallus pollen request Dear All:=C2=A0 I am in Ecuador with poor connections. Could someone please advise my old friend Boyce Tankersly how he can best save and share pollen of A. titanium with others? Tiom -----Original Message----- From: Boyce Tankersley [mailto:btankers@chicagobotanic.org] Sent: Wednesday, August 12, 2015 4:32 PM To: Tom Croat Subject: Amorphophallus pollen request Hi Tom: The Amorphophallus titanum at Chicago Botanic Garden is coming into bloom and we would like to collect pollen to share with other institutions.=C2=A0 Can you provide any insight into the best approach to take and store (long term) the pollen? Many thanks, Boyce Tankersley Director of Living Plant Documentation Chicago Botanic Garden 1000 Lake Cook Road Glencoe, IL 60022 Email: btankers@chicagobotanic.org Phone: 847-835-6841 Fax: 847-835-1635 -----Original Message----- From: Nancy Ruth Morin [mailto:Nancy.Morin@NAU.EDU] Sent: Wednesday, August 12, 2015 12:00 PM To: AABGACOL@LIST.MSU.EDU Subject: Re: Amorphophallus pollen request Hi Boyce!=C2=A0 You might ask Tom Croat at MO at least about the pollen collecting. Nancy Morin Flora of North America Business Office P. O. Box 716 Point Arena, CA 95468 707/882-2528 ________________________________________ From: Boyce Tankersley [btankers@CHICAGOBOTANIC.ORG] Sent: Wednesday, August 12, 2015 9:51 AM To: AABGACOL@LIST.MSU.EDU Subject: Amorphophallus pollen request Greetings: The Amorphophallus titanum at CBG is about to come into flower and we would like to pollinate the flowers.=C2=A0 Does anyone have pollen that they can share with us?=C2=A0 Full bloom in a couple of days or so. Related request, does anyone have experience collecting pollen from Amorphophallus that they could share? Many thanks, Boyce Tankersley Director of Living Plant Documentation Chicago Botanic Garden 1000 Lake Cook Road Glencoe, IL 60022 Email: btankers@chicagobotanic.org<mailto:btankers@chicagobotanic.org> Phone: 847-835-6841 Fax: 847-835-1635 _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l --001a1149369ea5d1cd051d8812be-- --===============1979477537369440296==

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