Propogation of Amorphophallus from Petiole Tissue

From: Don Burns <burns at mobot.mobot.org> on 1999.04.08 at 02:21:07(3244) We have periodically discussed the propogation of Amorphophallus titanum (I believe) from leaf tissue. Has anyone tried this with tissue from petioles of any of the A. species? Don +More Don Burns Plantation, FL USA Zone 10b

From: Gijs Grob <Grob at rulrhb.leidenuniv.nl> on 1999.04.08 at 13:40:30(3246) >We have periodically discussed the propogation of Amorphophallus titanum >(I believe) from leaf tissue. Has anyone tried this with tissue from >petioles of any of the A. species? > >Don > > >Don Burns Plantation, FL USA Zone 10b Dear Don, For my DNA-research I have to cut Amorphophallus leaflets . Just for fun I kept some of these leaflets in water to see how long they will stay green. Now I have got leaflets of three Amorphophallus species, which are green since october and have rapidly developed small tubers with roots. I do not know whether they will survive a transport into soil, for they are still in water. Gijs +More --------------------------------------------------------------- Gijs Grob Rijksherbarium/Hortus Botanicus, University of Leiden Bijzondere plantkunde, University of Amsterdam Kruislaan 318 1098 SM Amsterdam The Netherlands Tel: (+31.20) 525 7821 /7823 E-mail: grob@rulrhb.leidenuniv.nl

From: "Mr R.a McClure" <Rob.McClure at sci.monash.edu.au> on 1999.04.08 at 13:44:01(3247) > We have periodically discussed the propogation of Amorphophallus titanum > (I believe) from leaf tissue. Has anyone tried this with tissue from > petioles of any of the A. species? > > Don Burns Plantation, FL USA Zone 10b Don, I have tried it on Amorph. galbra and it failed miserably. Undeterred I have done another batch of galbra and some variablis. They are looking ok so far and I am hoping my new whizz-bang heating pad is going to do the trick. Cheers, Rob. +More Rob.McClure Biological Sciences Monash University, Clayton 3168 Victoria AUSTRALIA. Phone:(03)9905.5625 Fax:(03)9905.5613 e-mail: Rob.McClure@sci.monash.edu.au

From: "Julius Boos" <ju-bo at email.msn.com> on 1999.04.08 at 13:47:55(3248) >We have periodically discussed the propogation of Amorphophallus titanum (I believe) from leaf tissue. Has anyone tried this with tissue from petioles of any of the A. species? Don< Dear Don, My friend Bobby Mc Gehe here in W.P.B. called me and showed me an Amorphophallus Sp. that had re-generated after it`s petiole had accidently broken off cleanly about 1" above the tuber, he had just stuck the petiole back into his potting medium, and it rooted. It MAY have been A, albispathus, but I can not be sure. I believe I wrote to 'King Amorphohallus' Wilbert and reported it at the time, but again can not be sure! This was several years ago. Cheers, Julius +More Don Burns Plantation, FL USA Zone 10b

From: Wilbert Hetterscheid <hetter at vkc.nl> on 1999.04.09 at 13:57:46(3252) King here! Yes, Julius story has been repeated here. I also once had a broken leaf and the petiole regenerated a tuber at the base and is now growing fine. Mind you, I am sure not all species will do this but the ones with very turgid petioles will (and now all of you wann know which ones I mean, huh? Well, figure it out yourselves...... I am not going to demolish all leaves that are up now.....). Gijs' story is remarkable but represents the same phenomenon. Stress apparently can induce tuber generation. Who knows, Gijs, maybe these are retrotransposons that are activated by stress and do their thing........(this was just showing-off with terms.......). Cheers all, Wilbert +More > ---------- > From: Julius Boos[SMTP:ju-bo@email.msn.com] > Reply To: aroid-l@mobot.org > Sent: donderdag 8 april 1999 15:46 > To: hetter@vkc.nl > Subject: Re: Propogation of Amorphophallus from Petiole Tissue > > -----Original Message----- > From: Don Burns > To: ju-bo@msn.com > Date: Wednesday, April 07, 1999 10:21 PM > Subject: Propogation of Amorphophallus from Petiole Tissue > > > >We have periodically discussed the propogation of Amorphophallus > titanum > (I believe) from leaf tissue. Has anyone tried this with tissue from > petioles of any of the A. species? > > Don< > > Dear Don, > My friend Bobby Mc Gehe here in W.P.B. called me and showed me an > Amorphophallus Sp. that had re-generated after it`s petiole had > accidently > broken off cleanly about 1" above the tuber, he had just stuck the > petiole > back into his potting medium, and it rooted. It MAY have been A, > albispathus, but I can not be sure. I believe I wrote to 'King > Amorphohallus' Wilbert and reported it at the time, but again can not > be > sure! This was several years ago. > Cheers, > Julius > > > Don Burns Plantation, FL USA Zone 10b > > > > > >

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