6-benzylamino purine (question)

From: mburack at mindspring.com on 2001.05.22 at 19:57:50(6532) Has anyone ever used 6-BA to stimulate seed germination? If so, please let me some details. It can be purchased in a powder form, so info on dilution, frequency, use etc.. would be very helpful.. (someone on the list-serv must be familiar with this??) Thanks in advance Marc Burack

From: StroWi at t-online.de (StroWi) on 2001.05.22 at 22:00:47(6534) mburack@mindspring.com schrieb: > > Has anyone ever used 6-BA to stimulate seed germination? > > If so, please let me some details. It can be purchased in a powder form, so > info on dilution, frequency, use etc.. would be very helpful.. > > (someone on the list-serv must be familiar with this??) > > Thanks in advance > > Marc Burack +More Marc, I have not used 6-BA to stimulate seed germination or have done any other in vivo application, but I have used it in tissue culture of various plant species. 6-BA is a synthetic cytokinin that is normally used to break/reduce apical dominance in shoot cultures or to stimulate outgrowth of (dormant) buds for example in node cultures. Further it can induce adventitious shoots, that is that new shoot meristems are regenerated and can grow on to build normal shoots with an apex and lateral buds etc. (BTW, it is often used in combination with an auxin in low concentration) A side effect of cytokinins is often that they inhibit rooting, more precisely regeneration of adventitious roots due to a direct effect or due to accumulation of metabolites. So you might stimulate shoot growth in seeds/seedlings, but might inhibit normal root growth. 6-BA is of medium strength; for example Kinetin is weaker and Thidiazuron (TDZ) is much stronger. It is used in concentrations of less than 0,1 mg/liter to 10,0 mg/liter or more; for example in Phalenopsis shoot cultures 2,0 mg/liter is often used. 6-BA can be dissolved in 1 n HCl or in 1 n KOH and be diluted with H2O to make a (stock) solution of 0,5 or 1,0 mg/milliliter. I heard of in vivo applications of cytokinins, but don't know any details (concentration, application frequency etc.). But I remember, that unwanted side effects were noticed, for example inhibition of flowering or malformed habitus etc. So, one might be careful in regard of concentration and application frequency etc. Hope this helps in some way, Bernhard Strolka.

From: StroWi at t-online.de (StroWi) on 2001.05.23 at 05:41:26(6540) mburack@mindspring.com schrieb: > > Has anyone ever used 6-BA to stimulate seed germination? > > If so, please let me some details. It can be purchased in a powder form, so > info on dilution, frequency, use etc.. would be very helpful.. > > (someone on the list-serv must be familiar with this??) > Marc, See also at http://www.aroid.org/ Aroid Horticultural Links +More by Scott Hyndman General Growing Information A Review of Literature Concerning the Use of Growth Regulators to Induce Lateral or Basal Shoot Production Hope this helps,too, Bernhard Strolka. > Thanks in advance > > Marc Burack

From: Scott Hyndman hyndman at aroid.org> on 2001.05.23 at 15:13:49(6541) Marc, In addition to the good information that Bernhard Strolka gave us on BA use, especially the in vitro application, BA was used commercially by aroid foliage plant liner producers back in the early 90s to promote axillary shoot (sucker) growth to get a more full liner. The hormone was very inexpensive then and gave fairly good results. Jake Henny of the University of Florida, Mid Florida Research and Education Center, did much of the research on this application. If you are specifically interested in stimulating seed germination, you will want to investigate the use of gibberellic acid (GA). GA3 is usually commercially available, and it is usually inexpensive. There is some information on the Web at http://koning.ecsu.ctstateu.edu/Plants_human/seedgerm.html and at http://www.crfg.org/tidbits/gibberellic.html. Another research area that Jake Henny has worked on is the use of GA in stimulating the flowering of aroids, an important technique useful in hybridizing. Regards, Scott +More --------------------- Mr. Scott Hyndman International Aroid Society Web Site Vero Beach, Florida, USA USDA Hardiness Zone 10a E-mail: hyndman@aroid.org on 5/22/01 3:57 PM, mburack@mindspring.com at mburack@mindspring.com wrote: > > Has anyone ever used 6-BA to stimulate seed germination? > > If so, please let me some details. It can be purchased in a powder form, so > info on dilution, frequency, use etc.. would be very helpful.. > > (someone on the list-serv must be familiar with this??) > > Thanks in advance > > Marc Burack

From: mburack at mindspring.com on 2001.05.23 at 15:17:45(6545) Dear Bernhard, Many thanks for the reply, but I think I should ask my question a little easier, so that I may understand your answer (smile). (I am definately looking for the less technical kind of info :-) I was told there was a way to make a solution with this chemical, and it could be sprayed onto "ungerminated" seed. The story was that the reaction would cause seed to germinate very quickly. Does this sound right to you? If so how would you do it? Thanks again in advance. +More aroid-l@mobot.org wrote: > mburack@mindspring.com schrieb: > > Has anyone ever used 6-BA to stimulate seed germination? > > If so, please let me some details. It can be purchased in a powder form, so > info on dilution, frequency, use etc.. would be very helpful.. > > (someone on the list-serv must be familiar with this??) > Marc, See also at http://www.aroid.org/ Aroid Horticultural Links by Scott Hyndman General Growing Information A Review of Literature Concerning the Use of Growth Regulators to Induce Lateral or Basal Shoot Production Hope this helps,too, Bernhard Strolka. > Thanks in advance > > Marc Burack

From: mburack at mindspring.com on 2001.05.24 at 14:23:55(6552) Dear Scott, Based on the info you sent, I think that GA is really more in line what I am looking for.... I purchased some yesterday although it seemed more expensive than I thought... I paid $25 for 1 gram. I really dont know if that is expensive or not, but obviously since I am going to use small amounts per application, 1 gram should be good for anywhere from 5-8 uses. I am hoping the results are worth it....I will keep you informed. Thanks again! +More aroid-l@mobot.org wrote: > Marc, In addition to the good information that Bernhard Strolka gave us on BA use, especially the in vitro application, BA was used commercially by aroid foliage plant liner producers back in the early 90s to promote axillary shoot (sucker) growth to get a more full liner. The hormone was very inexpensive then and gave fairly good results. Jake Henny of the University of Florida, Mid Florida Research and Education Center, did much of the research on this application. If you are specifically interested in stimulating seed germination, you will want to investigate the use of gibberellic acid (GA). GA3 is usually commercially available, and it is usually inexpensive. There is some information on the Web at http://koning.ecsu.ctstateu.edu/Plants_human/seedgerm.html and at http://www.crfg.org/tidbits/gibberellic.html. Another research area that Jake Henny has worked on is the use of GA in stimulating the flowering of aroids, an important technique useful in hybridizing. Regards, Scott --------------------- Mr. Scott Hyndman International Aroid Society Web Site Vero Beach, Florida, USA USDA Hardiness Zone 10a E-mail: hyndman@aroid.org on 5/22/01 3:57 PM, mburack@mindspring.com at mburack@mindspring.com wrote: > > Has anyone ever used 6-BA to stimulate seed germination? > > If so, please let me some details. It can be purchased in a powder form, so > info on dilution, frequency, use etc.. would be very helpful.. > > (someone on the list-serv must be familiar with this??) > > Thanks in advance > > Marc Burack

From: StroWi at t-online.de (StroWi) on 2001.05.28 at 02:45:58(6585) Scott, thanks for your information! Scott Hyndman schrieb: +More > Marc, > > In addition to the good information that Bernhard Strolka gave us on BA use, > especially the in vitro application, BA was used commercially by aroid > foliage plant liner producers back in the early 90s to promote axillary > shoot (sucker) growth to get a more full liner. The hormone was very > inexpensive then and gave fairly good results. Jake Henny of the University > of Florida, Mid Florida Research and Education Center, did much of the > research on this application. Would you know where to find papers or other detailed information on Jake Henny's research? > > If you are specifically interested in stimulating seed germination, you will > want to investigate the use of gibberellic acid (GA). Has GA3 been tried on (dormant) tubers as well? I would be intersested in any details or publications.... Thanks, Bernhard.

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