Pinellia hybrid.... and others...
> From: James Waddick
> To: Discussion of aroids
href="mailto:aroid-l@www.gizmoworks.com">aroid-l@www.gizmoworks.com
/>....
> In this same discussion, the cv
"Indian Giant' is brought to
> mind. This is a much larger
form of S. venosum. Could it be a
> tetraploid? Has anyone
compared chromosome numbers to the typical,
> smaller form? And
is this, by the way, any more or less hardier than
> the typical
form?
Dear Jim W.,
style="margin:0px;">Aroiders,
already
more than one year ago I compared the ploidy level of a normal form of
Typhonium venosum (a genotype/tuber that came from Laos) with my 'Indian
Giant' clone that was originally introduced by Tony Avent (Plant
Delights Nursery). Furthermore I have the clone that was given to me as
T. nubicum. but is probably identical with 'Indian Giant'.
style="margin:0px;">For pictures see: (scroll down to the post from
06.11.2009)
href="http://www.amorphophallus-forum.de/viewtopic.php?f=9&t=316&start=30">http://www.amorphophallus-forum.de/viewtopic.php?f=9&t=316&start=30
/>
I did not count the chromosomes but used
flowcytometry to compare the DNA content.
In
short words with this method the DNA content of nuclei is measured after
the DNA was stained.
The measured values are
shown in a graph.
The first peak represents
the cells in the G1-phase; this would give the DNA-content of somatic
cells. The second peak represents the nucelar DNA content of cell in the
G2-phase after the replication of the DNA before the cell
devides.
The graphs show the
measurements of T. venosum 'Laos', T.venosum 'Indian Giant' and T.
nubicum, respectively.
class="MsoNormal" style="margin:0px;">
class="MsoNormal" style="margin:0px;">[img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_v_Laos.jpg[/img]
/>
class="MsoNormal" style="margin:0px;">[img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_v_IndianGiant.jpg[/img]
style="MARGIN: 0cm 0cm 0pt" class="MsoNormal" style="margin:0px;">[img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_nubicum.jpg[/img]
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class="MsoNormal" style="margin:0px;">
style="margin:0px;">The results strongly indicate that the three clones
do not differ in DNA content and therefore have the same ploidy
level.
class="MsoNormal" style="margin:0px;">
style="margin:0px;">However, I did no chomosome counts, but if 'Indian
Giant' would be a tetraploid of the normal form of T. venosum, the graph
should show a first peak at channel 100 for the G1-nuclei and a second
at channel 200 for the G2-nulei. This is not the case; all three clones
have the peaks roughly at channel 50 and channel
100.
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/>
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style="MARGIN: 0cm 0cm 0pt" class="MsoNormal" style="margin:0px;">
Best,
Bernhard.
--==============A91384668144448325= | 
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