Hybrids and doubling chromosomes

From: Brian Williams pugturd at alltel.net> on 2003.11.21 at 23:23:27(10846) I am interested in hearing if anyone has been doing hybrid work with any Aroids. Extremly interested in the storing of pollen in freezers or other ways. Would be interested in exact temps time periods and results. I have also heard of Hybrid amorphophallus though never seen a pic. Does this thing exist and if so why is their no info or pics of it. I understand the botanist point of view but from a collectors its very interesting. +More Last but not least has anyone tried chromosome doubling? Using chemicals on aroids like Colchincine? I have been reading up on it. It seems very interesting. Has anyone ever tried or working with this or other types of chemicals to produce tetraploids? THANKS

From: plantguy at zoominternet.net> on 2003.11.22 at 00:50:32(10848) Hi Brian, Sounds like an interesting project. We use colchicine all the time to disrupt microtubules and look at their role in vesicular trafficking of ion channels in my lab, but I never before looked into it in terms of plants. After reading your post and then a few articles I guess I'd say what the heck, it's so easy why not give it a whirl. Obviously, all you'd need is enough seed/plant material to try many concentrations and various time points. I would caution that if you don't know what your doing with toxins like this it wouldn't be a wise choice. Hopefully you have a good analytical balance and a hood for working with these types of compounds. If you're planning on using DMSO as a carrier I'd hope you would double glove....better safe than sorry. Perhaps this would be a fun project for next spring in the lab. It will be interesting to see what others familiar with plant cell biology have to say! Dan Devor +More ----- Original Message ----- To: Sent: Friday, November 21, 2003 6:23 PM Subject: [aroid-l] Hybrids and doubling chromosomes > I am interested in hearing if anyone has been doing hybrid work with any > Aroids. Extremly interested in the storing of pollen in freezers or > other ways. Would be interested in exact temps time periods and results. > > I have also heard of Hybrid amorphophallus though never seen a pic. > Does this thing exist and if so why is their no info or pics of it. I > understand the botanist point of view but from a collectors its very > interesting. > > Last but not least has anyone tried chromosome doubling? Using chemicals > on aroids like Colchincine? I have been reading up on it. It seems very > interesting. Has anyone ever tried or working with this or other types > of chemicals to produce tetraploids? > > THANKS > > >

From: lists at gingerwoodnursery.com on 2003.11.22 at 19:03:11(10851) Brian, You might want to use treflan instead of colchicine. As far as creating tetraploids specifically, it would be much more efficient and safer. Colchicine has its uses too, but treflan would be your best bet. Can forward a link on procedures if you need it. Don't know if its been tried on aroids or not, but don't see why it wouldn't work. Tim C. +More >-- Original Message -- >From: >To: >Subject: Re: [aroid-l] Hybrids and doubling chromosomes >Date: Fri, 21 Nov 2003 19:50:32 -0500 >Reply-To: aroid-l@lists.ncsu.edu > > >Hi Brian, > >Sounds like an interesting project. We use colchicine all the time to >disrupt microtubules and look at their role in vesicular trafficking of ion >channels in my lab, but I never before looked into it in terms of plants. >After reading your post and then a few articles I guess I'd say what the >heck, it's so easy why not give it a whirl. Obviously, all you'd need is >enough seed/plant material to try many concentrations and various time >points. I would caution that if you don't know what your doing with toxins >like this it wouldn't be a wise choice. Hopefully you have a good >analytical balance and a hood for working with these types of compounds. > If >you're planning on using DMSO as a carrier I'd hope you would double >glove....better safe than sorry. Perhaps this would be a fun project for >next spring in the lab. It will be interesting to see what others familiar >with plant cell biology have to say! > >Dan Devor > > >----- Original Message ----- >From: "Brian Williams" >To: >Sent: Friday, November 21, 2003 6:23 PM >Subject: [aroid-l] Hybrids and doubling chromosomes > > >> I am interested in hearing if anyone has been doing hybrid work with any >> Aroids. Extremly interested in the storing of pollen in freezers or >> other ways. Would be interested in exact temps time periods and results. >> >> I have also heard of Hybrid amorphophallus though never seen a pic. >> Does this thing exist and if so why is their no info or pics of it. I >> understand the botanist point of view but from a collectors its very >> interesting. >> >> Last but not least has anyone tried chromosome doubling? Using chemicals >> on aroids like Colchincine? I have been reading up on it. It seems very >> interesting. Has anyone ever tried or working with this or other types >> of chemicals to produce tetraploids? >> >> THANKS >> >> >> > >

From: "zach" r2ot at charter.net> on 2003.11.22 at 23:49:26(10852) hello How many chromsone copies would most aroids naturally have +More -zach

From: "C. J. Addington" cjaddington at earthlink.net> on 2003.11.24 at 01:33:30(10853) on 11/21/03 15:23, Brian Williams at pugturd@alltel.net wrote: > I am interested in hearing if anyone has been doing hybrid work with any > Aroids. Extremly interested in the storing of pollen in freezers or > other ways. Would be interested in exact temps time periods and results. > > I have also heard of Hybrid amorphophallus though never seen a pic. > Does this thing exist and if so why is their no info or pics of it. I > understand the botanist point of view but from a collectors its very > interesting. +More > > Last but not least has anyone tried chromosome doubling? Using chemicals > on aroids like Colchincine? I have been reading up on it. It seems very > interesting. Has anyone ever tried or working with this or other types > of chemicals to produce tetraploids? > > THANKS > Hi Brian! I have successfully pollinated Dracunculus using frozen pollen, so I know it can survive the freezing. I just put pollen-coated carrion beetles in a clean glass jar and covered it with foil. When I later dump the frozen beetles into freshly opened blooms, they set seed quite well. I have only stored the pollen for a couple of weeks, waiting for a good bloom to open, so I am not sure how long the pollen would last. I would think that if you kept it dry and didn't subject it to fluctuating temperatures, it should last quite a long time. CJ Addington

From: Thomas.Croat at mobot.org on 2003.12.01 at 19:53:54(10867) Dear Zach: Are you referring to Chromosome number? In the diploid condition there is quite a lot of variation, including 12, 20, 30 but 30 is a very common chromozome number in most genera. There are papers which survey the chromosome numbers for the whole family. Petersen, G. 1989. Cytology, systematics and chromosome numbers of Araceae. Nord. J. Bot. 9: 119-166. +More ___________. 1993. New chromosome numbers in Araceae. Willdenowia 23: 239-244. ___________. 1993b. Chromosome numbers of the genera of Araceae. Aroideana 16: 37-46. Tom -----Original Message----- Sent: Saturday, November 22, 2003 5:49 PM To: aroid-l@lists.ncsu.edu Subject: Re: [aroid-l] Hybrids and doubling chromosomes hello How many chromsone copies would most aroids naturally have -zach

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