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  Hybrids and doubling chromosomes
From: Brian Williams pugturd at alltel.net> on 2003.11.21 at 15:23:27(10846)
I am interested in hearing if anyone has been doing hybrid work with any
Aroids. Extremly interested in the storing of pollen in freezers or
other ways. Would be interested in exact temps time periods and results.

I have also heard of Hybrid amorphophallus though never seen a pic.
Does this thing exist and if so why is their no info or pics of it. I
understand the botanist point of view but from a collectors its very
interesting.

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From: plantguy at zoominternet.net> on 2003.11.21 at 16:50:32(10848)
Hi Brian,

Sounds like an interesting project. We use colchicine all the time to
disrupt microtubules and look at their role in vesicular trafficking of ion
channels in my lab, but I never before looked into it in terms of plants.
After reading your post and then a few articles I guess I'd say what the
heck, it's so easy why not give it a whirl. Obviously, all you'd need is
enough seed/plant material to try many concentrations and various time
points. I would caution that if you don't know what your doing with toxins
like this it wouldn't be a wise choice. Hopefully you have a good
analytical balance and a hood for working with these types of compounds. If
you're planning on using DMSO as a carrier I'd hope you would double
glove....better safe than sorry. Perhaps this would be a fun project for
next spring in the lab. It will be interesting to see what others familiar
with plant cell biology have to say!

Dan Devor

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From: lists at gingerwoodnursery.com on 2003.11.22 at 11:03:11(10851)
Brian,

You might want to use treflan instead of colchicine. As far as creating
tetraploids specifically, it would be much more efficient and safer. Colchicine
has its uses too, but treflan would be your best bet. Can forward a link
on procedures if you need it. Don't know if its been tried on aroids or
not, but don't see why it wouldn't work.

Tim C.

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From: "zach" r2ot at charter.net> on 2003.11.22 at 15:49:26(10852)
hello

How many chromsone copies would most aroids naturally
have

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From: "C. J. Addington" cjaddington at earthlink.net> on 2003.11.23 at 17:33:30(10853)
on 11/21/03 15:23, Brian Williams at pugturd@alltel.net wrote:

> I am interested in hearing if anyone has been doing hybrid work with any
> Aroids. Extremly interested in the storing of pollen in freezers or
> other ways. Would be interested in exact temps time periods and results.
>
> I have also heard of Hybrid amorphophallus though never seen a pic.
> Does this thing exist and if so why is their no info or pics of it. I
> understand the botanist point of view but from a collectors its very
> interesting.

+More
From: Thomas.Croat at mobot.org on 2003.12.01 at 11:53:54(10867)
Dear Zach:

Are you referring to Chromosome number? In the diploid condition
there is quite a lot of variation, including 12, 20, 30 but 30 is a very
common chromozome number in most genera. There are papers which survey the
chromosome numbers for the whole family.

Petersen, G. 1989. Cytology, systematics and chromosome numbers of
Araceae. Nord. J. Bot. 9: 119-166.

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