Re: [aroid-l] Hybrids and doubling chromosomes

From: plantguy at zoominternet.net> on 2003.11.22 at 00:50:32(10848) Hi Brian, Sounds like an interesting project. We use colchicine all the time to disrupt microtubules and look at their role in vesicular trafficking of ion channels in my lab, but I never before looked into it in terms of plants. After reading your post and then a few articles I guess I'd say what the heck, it's so easy why not give it a whirl. Obviously, all you'd need is enough seed/plant material to try many concentrations and various time points. I would caution that if you don't know what your doing with toxins like this it wouldn't be a wise choice. Hopefully you have a good analytical balance and a hood for working with these types of compounds. If you're planning on using DMSO as a carrier I'd hope you would double glove....better safe than sorry. Perhaps this would be a fun project for next spring in the lab. It will be interesting to see what others familiar with plant cell biology have to say! Dan Devor +More ----- Original Message ----- To: Sent: Friday, November 21, 2003 6:23 PM Subject: [aroid-l] Hybrids and doubling chromosomes > I am interested in hearing if anyone has been doing hybrid work with any > Aroids. Extremly interested in the storing of pollen in freezers or > other ways. Would be interested in exact temps time periods and results. > > I have also heard of Hybrid amorphophallus though never seen a pic. > Does this thing exist and if so why is their no info or pics of it. I > understand the botanist point of view but from a collectors its very > interesting. > > Last but not least has anyone tried chromosome doubling? Using chemicals > on aroids like Colchincine? I have been reading up on it. It seems very > interesting. Has anyone ever tried or working with this or other types > of chemicals to produce tetraploids? > > THANKS > > >

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