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  Tissue Culture ? Can common folk do it?
From: santoury at aol.com on 2010.09.09 at 14:54:00(21426)
I'm sure this has been answered before, but nothing on the internet shows any "common folk" doing tissue culture work to propagate their own plants. I'd love to do it, if possible. Granted, my facilities are not "scientific." But, boy, if I could grow plants from pieces of leaves - I'd sure love to.
I'd love to hear about any experience you may have had with this endeavor.

Thanks! Jude

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From: "plantguy at zoominternet.net" <plantguy at zoominternet.net> on 2010.09.09 at 16:09:55(21428)
Hi Jude,

I must admit, I have often thought of trying it in my lab which is complete
with anything that would be necessary, but have never found the time or
actual inclination to have a stab at it.

There are a lot of sites on the internet that show you how to do this as a
home-TC person however. Sterility will be the biggest problem, but can be
solved with 70% EtOH and a home-made hood.....also a alcohol burner. I've
always thought the biggest issue would be which formulation to make for the
agar you are doing the TC on as there are a million possible supplements
that can be added (the agar also needs to be sterilized which is typically
done in an autoclave).

Best of luck if you give it a try and I would be interested in hearing your
results.

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From: Don Martinson <llmen at wi.rr.com> on 2010.09.09 at 16:57:10(21430)
In general, tissue culture is a very exacting and not inexpensive endeavor and not really intended for (no offense intended) us “common folk”. I’m not saying it can’t be done, but I don’t believe it is easily achieved without specialized materials and lots of expert help.

Don Martinson

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From: "Sherry Gates" <TheTropix at msn.com> on 2010.09.09 at 17:33:13(21431)
I excitedly 2nd this request!

thanks, sherry

----- Original Message -----

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From: "StroWi at t-online.de" <StroWi at t-online.de> on 2010.09.09 at 22:31:01(21433)
Jude,

there is a yahoo group on hometissueculture...
http://tech.groups.yahoo.com/group/hometissueculture/

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From: Zach DuFran <zdufran at wdtinc.com> on 2010.09.10 at 06:55:21(21435)
Jude-

One of our IAS members (Ghazanfar Ghori) has a very nice
blog where he documents his work with Cryptocorynes. He does a lot of
tissue culture. Check it out:

http://kryptokoryne.aquaticscape.com/

Zach

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From: Steve Marak <samarak at gizmoworks.com> on 2010.09.11 at 10:29:35(21443)
My wife's setup involves a pressure cooker, an electronic kitchen balance
that reads in grams, and a "hood" made by buying a large clear plastic bin
at Wal-Mart, turning it upside down, and cutting a hole in the side and
taping a clear plastic flap over it. After trying several options, she's
now using regular home canning jars (with the plastic lids, not the metal
ones) to hold the medium.

We happened to already have the pressure cooker and electronic kitchen
balance, but other than those, the most expensive thing has been
purchasing the media, which Cathy gets from PhytoTech (
http://www.phytotechlab.com ). She's buying pre-mixed formulations they
offer, which require only the addition of sugar.

At the moment she's focused on learning to flask orchid seed efficiently,
rather than tissue culture, but she has done a little TC in that kind of
setup when she was teaching high school, so it can be done.

Carolina Biological ( http://www.carolina.com ) offers TC "kits" designed
for teachers/students/home enthusiasts (put "tissue culture" in the search
box). They won't set you up to do TC of your plants at home, but can give
you a feel for the kind of lab techniques you need (and help give you the
confidence that you can do it), and several of them are pretty cheap - the
sundew TC kit is less than $25 US.

Sterilization of the environment in the hood is key, of course. Some
people use a spray bottle of ethanol, but not only is it very "fumey",
it's very flammable, so Cathy uses other options. You can use dilute
bleach, or some people prefer a calcium hypochlorite (household bleach is
a sodium hypochlorite solution) solution instead. Aaron Hicks, at his
Orchid Seedbank Project, has lots of great advice from his experience
flasking orchids which would apply in TC also. Check out
http://members.cox.net/ahicks51/osp/

Steve

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From: santoury at aol.com on 2010.09.11 at 14:01:20(21445)
Hey there
Thank you very much - Here I was thinking, hoping, that it would just be a matter of cutting up some leaves, and putting them on agar... The whole spraying, chemicals and such - no thanks! Darn. One could only hope, right? :)

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From: "Steve Hatfield" <moondogman at comcast.net> on 2010.09.11 at 14:11:37(21446)
http://www.hometissueculture.org/

I found this a few months ago.It looks doable on a budget. I think a
tinkerer could come up with most of the things in the kits.

Check out these videos on YouTube Micropropagation Part I Intro.

http://www.youtube.com/watch?v=KYvJByYrSPg

looks doable.

Steve

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From: "plantguy at zoominternet.net" <plantguy at zoominternet.net> on 2010.09.11 at 14:12:56(21447)
Hi Steve,

Sounds like your wife is having some fun :o)

I guess I forgot about a nice place to look up info and get a free book on
the subject. If you go to www.sigma-aldrich.com you can find a huge number
of things by putting plant tissue culture in the search box. The only
problem is that you can get overwhelmed by the sheer volume of
info.....beware if you really want scientific info.....there is a massive
amount of it.

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From: "Nancy Greig" <ngreig at hmns.org> on 2010.09.12 at 07:26:26(21453)
I seem to recall that Steve Lucas mentioned to me that Tony Avent had written an article for the IAS journal on growing Amorphophallus plants from leaf cuttings. Does anyone know the issue number?

Nancy Greig

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From: ExoticRainforest <Steve at ExoticRainforest.com> on 2010.09.12 at 14:42:38(21454)
Nancy, it is in volume 30.

Steve

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From: "Derek Burch" <derek at horticulturist.com> on 2010.09.12 at 19:16:51(21457)
Nancy,

There is a searchable index on Aroid-l (saved me going to the files).

2007 30 124-138 Tony Avent Propagation of Amorphophallus by
leaf petiole cuttings (Buy)

He takes away a lot of the mystery.

I haven't looked back in the Aroid-l archives, but this topic or one like it
has come up in the past. It is worth getting to know all the things on
aroid-l: Aroideana stuff, aroiders, cultivar registration, and many more
gems, not to mention great galleries of images. It is not limited to members
of IAS, although we all hope that it generates membership.

Derek

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