Re: [Aroid-l] Tissue Culture ? Can common folk do it?

From: "Derek Burch" <derek at horticulturist.com> on 2010.09.13 at 02:16:51(21457) Nancy, There is a searchable index on Aroid-l (saved me going to the files). 2007 30 124-138 Tony Avent Propagation of Amorphophallus by leaf petiole cuttings (Buy) He takes away a lot of the mystery. I haven't looked back in the Aroid-l archives, but this topic or one like it has come up in the past. It is worth getting to know all the things on aroid-l: Aroideana stuff, aroiders, cultivar registration, and many more gems, not to mention great galleries of images. It is not limited to members of IAS, although we all hope that it generates membership. Derek +More -----Original Message----- From: aroid-l-bounces@www.gizmoworks.com [mailto:aroid-l-bounces@www.gizmoworks.com] On Behalf Of Nancy Greig Sent: Sunday, September 12, 2010 10:26 AM To: Discussion of aroids Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it? I seem to recall that Steve Lucas mentioned to me that Tony Avent had written an article for the IAS journal on growing Amorphophallus plants from leaf cuttings. Does anyone know the issue number? Nancy Greig Director, Cockrell Butterfly Center Houston Museum of Natural Science One Hermann Circle Drive Houston, TX 77030-1799 tel 713-639-4742 fax 713-639-4788 www.hmns.org -----Original Message----- From: aroid-l-bounces@www.gizmoworks.com on behalf of santoury@aol.com Sent: Sat 9/11/2010 4:01 PM To: aroid-l@www.gizmoworks.com Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it? Hey there Thank you very much - Here I was thinking, hoping, that it would just be a matter of cutting up some leaves, and putting them on agar... The whole spraying, chemicals and such - no thanks! Darn. One could only hope, right? :) -----Original Message----- From: Steve Marak To: Discussion of aroids Sent: Sat, Sep 11, 2010 1:29 pm Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it? My wife's setup involves a pressure cooker, an electronic kitchen balance that reads in grams, and a "hood" made by buying a large clear plastic bin at Wal-Mart, turning it upside down, and cutting a hole in the side and taping a clear plastic flap over it. After trying several options, she's now using regular home canning jars (with the plastic lids, not the metal ones) to hold the medium. We happened to already have the pressure cooker and electronic kitchen balance, but other than those, the most expensive thing has been purchasing the media, which Cathy gets from PhytoTech ( http://www.phytotechlab.com ). She's buying pre-mixed formulations they offer, which require only the addition of sugar. At the moment she's focused on learning to flask orchid seed efficiently, rather than tissue culture, but she has done a little TC in that kind of setup when she was teaching high school, so it can be done. Carolina Biological ( http://www.carolina.com ) offers TC "kits" designed for teachers/students/home enthusiasts (put "tissue culture" in the search box). They won't set you up to do TC of your plants at home, but can give you a feel for the kind of lab techniques you need (and help give you the confidence that you can do it), and several of them are pretty cheap - the sundew TC kit is less than $25 US. Sterilization of the environment in the hood is key, of course. Some people use a spray bottle of ethanol, but not only is it very "fumey", it's very flammable, so Cathy uses other options. You can use dilute bleach, or some people prefer a calcium hypochlorite (household bleach is a sodium hypochlorite solution) solution instead. Aaron Hicks, at his Orchid Seedbank Project, has lots of great advice from his experience flasking orchids which would apply in TC also. Check out http://members.cox.net/ahicks51/osp/ Steve On Thu, 9 Sep 2010, Sherry Gates wrote: > I excitedly 2nd this request! > thanks, sherry > ----- Original Message ----- > From: santoury@aol.com > Subject: [Aroid-l] Tissue Culture ? Can common folk do it? > > I'm sure this has been answered before, but nothing on the internet > shows any "common folk" doing tissue culture work to propagate their > own plants. I'd love to do it, if possible. Granted, my facilities are > not "scientific." But, boy, if I could grow plants from pieces of > leaves - I'd sure love to. I'd love to hear about any experience you > may have had with this endeavor. Thanks! Jude -- Steve Marak -- samarak@gizmoworks.com _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l ------=_NextPart_000_0012_01CB52C8.339990B0 _______________________________________________ Aroid-L mailing list Aroid-L@www.gizmoworks.com http://www.gizmoworks.com/mailman/listinfo/aroid-l ------=_NextPart_000_0012_01CB52C8.339990B0--

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