Re: [aroid-l] A. Titanum cloned

From: "WEAVER,BILL (HP-USA,ex1)" bill.weaver at hp.com> on 2003.09.19 at 22:02:31(10608) I used just a leaflet one of the little (4" X 8") oval components that make up the entire leaf. I just took one that came to a nice point with very little leaf tissue on either side of the mid-rib where I cut it off. Bill +More -----Original Message----- Sent: Thursday, September 18, 2003 2:02 PM To: aroid-l@lists.ncsu.edu Subject: Re: [aroid-l] A. Titanum cloned Just so I am clear on this cloning technique: does one use the entire leaf, or trim the lateral material away from the midvein? And how much of the petiole is retained? Thanks dst D. Scott Taylor, Ph.D. Brevard County Environmentally Endangered Lands (EEL) Program Central Region Land Manager 5560 North US Highway 1 Melbourne, FL 32940 tel: 321.255.4466 FAX: 321.255.4499 email: staylor@brevardparks.com -----Original Message----- [mailto:aroid-l-owner@lists.ncsu.edu]On Behalf Of Douglas Ewing Sent: Thursday, September 18, 2003 11:36 AM To: 'aroid-l posting' Subject: Re: [aroid-l] A. Titanum cloned I too have been cloning titanum from leaf cuttings. We differ from Bill's procedure slightly: I use a 12" portion of the leaf with the mid-vein. I have not experimented with smaller cuttings. We apply no hormone. ( this is something I have avoided with all leaf cuttings in the past, as I was under the impression that auxin would promote root formation, but that it inhibits formation of shoots. Since we want both to happen with a leaf cutting, we do not apply. Tubers are shoots, perhaps this explains the difference in the gel vs. powder results?) We stick the cutting into a 8cm cube of rockwool, water, and encapsulate the entire affair in a terrarium made from 2 2liter pop-bottles. This creates almost total humidity, and cutting do not wilt. If/when fungus appears on the cutting, we dust with cinnamon. Tuber formation takes several months, then we wean from humidity by utilizing a pop-bottle with the bottom removed and the cap off, this allows a bit of air exchange for a few days , then the cutting is removed from the structure, potted up and placed on the open bench. Typically the orig. leaf tissue dies, followed by new leaf emergence. We also see multiple leaf emergence, which I am assuming will result in multiple tubers. I have not tried other sp. of this genus, but the fact that several species produce tubercles on the leaf naturally,( A. bulbifer) makes me think that all sp. might respond to this treatment. Doug _________________/\/\/\______________________ Doug Ewing, Greenhouse Manager (206) 543-0436 Department of Biology University of Washington Box 351800 Seattle, WA 98195-1800

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