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Avent
Plant Delights Nursery @
Juniper Level Botanic Garden
9241 Sauls Road
Raleigh, North
Carolina 27603
USA
Minimum Winter Temps 0-5 F
Maximum Summer Temps 95-105F
USDA Hardiness Zone 7b
email tony@plantdelights.com
website http://www.plantdelights.com
phone 919 772-4794
fax 919 772-4752
"I consider every plant hardy until I have killed it myself...at least
three times" - Avent
From:
aroid-l-bounces@www.gizmoworks.com [mailto:aroid-l-bounces@www.gizmoworks.com] On Behalf Of StroWi@t-online.de
Sent: Friday, January 21, 2011
9:07 AM
To: Discussion
of aroids
Subject: [Aroid-l] 'Indian Giant'
ploidylevel [was:Re: Sauromatum x Pinellia hybrid]
-----Original Message-----
> Date: Wed, 07 Jul 2010 21:03:55 +0200
> Subject: Re: [Aroid-l] Sauromatum x Pinellia hybrid.... and others...
> From: James Waddick
> To: Discussion of aroids
href="mailto:aroid-l@www.gizmoworks.com">aroid-l@www.gizmoworks.com
.....
> In this same discussion, the cv
"Indian Giant' is brought to
> mind. This is a much larger form of S. venosum. Could it be a
> tetraploid? Has anyone compared chromosome numbers to the typical,
> smaller form? And is this, by the way, any more or less hardier than
> the typical form?
Dear Jim W.,
Aroiders,
already more than one year ago I
compared the ploidy level of a normal form of Typhonium venosum (a genotype/tuber
that came from Laos)
with my 'Indian Giant' clone that was originally introduced by Tony Avent (Plant Delights Nursery). Furthermore I
have the clone that was given to me as T. nubicum. but is probably identical
with 'Indian Giant'.
For pictures see: (scroll down to
the post from 06.11.2009)
href="http://www.amorphophallus-forum.de/viewtopic.php?f=9&t=316&start=30">http://www.amorphophallus-forum.de/viewtopic.php?f=9&t=316&start=30
I did not count the chromosomes but
used flowcytometry to compare the DNA content.
In short words with this method
the DNA content of nuclei is measured after the DNA was stained.
The measured values are shown in a
graph.
The first peak represents the cells
in the G1-phase; this would give the DNA-content of somatic cells. The second
peak represents the nucelar DNA content of cell in the G2-phase after the
replication of the DNA before the cell devides.
The graphs show the measurements of
T. venosum 'Laos',
T.venosum 'Indian Giant' and T. nubicum, respectively.
[img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_v_Laos.jpg[/img]
[img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_v_IndianGiant.jpg[/img]
[img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_nubicum.jpg[/img]
The results strongly
indicate that the three clones do not differ in DNA content and therefore have
the same ploidy level.
However, I did no chomosome
counts, but if 'Indian Giant' would be a tetraploid of the normal form of T..
venosum, the graph should show a first peak at channel 100 for the
G1-nuclei and a second at channel 200 for the G2-nulei. This is not the case;
all three clones have the peaks roughly at channel 50 and channel 100.
Best, Bernhard.
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