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From: "StroWi@t-online.de"
Sender: "aroid-l-bounces@www.gizmoworks.com"
Date: Wed, 26 Jan 2011 01:05:21 -0500
To: Discussion of aroids
ReplyTo: Discussion of aroids
Subject: Re: [Aroid-l] 'Indian Giant' ploidylevel [was:Re: Sauromatum x
Pinellia hybrid]
Tony,
did you do selfings
of the F1 yet? If they are fertile, it would at least give another
indication that it's not triploid.
Happy
crossing, Bernhard.
-----Original
Message-----
> Date: Sun, 23 Jan 2011 15:13:30 +0100
>
Subject: Re: [Aroid-l] 'Indian Giant' ploidylevel [was:Re: Sauromatum
> x Pinellia hybrid]
> From: Tony Avent
> To: Discussion of aroids
>
Bernhard:
Thanks for
posting the results of your study of Sauromatum venosum. Since we
introduced S. ‘Indian Giant’ we have grown out several hundred crosses
between S. ‘Indian Giant’ and typical S. venosum. The resulting plants
are intermediate between both parents with an amazing amount of
variability including some plants with no spots on the petiole, and
others with an abundance of spots. Also, there is quite a bit of
difference with leaf size and leaf ruffling.
Tony Avent
Plant Delights Nursery @
Juniper
Level
Botanic Garden
9241 Sauls Road
Raleigh,
North Carolina
27603
USA
Minimum Winter Temps 0-5
F
Maximum Summer Temps 95-105F
USDA Hardiness Zone 7b
email tony@plantdelights.com
website http://www.plantdelights.com
phone 919 772-4794
fax 919 772-4752
"I consider every
plant hardy until I have killed it myself...at least three times" -
Avent
From:
aroid-l-bounces@www.gizmoworks.com
[mailto:aroid-l-bounces@www.gizmoworks.com] On Behalf Of StroWi@t-online.de
Sent: Friday, January 21, 2011 9:07
AM
To:
Discussion of aroids
Subject: [Aroid-l]
'Indian Giant' ploidylevel [was:Re: Sauromatum x Pinellia
hybrid]
-----Original
Message-----
> Date: Wed, 07 Jul 2010 21:03:55 +0200
>
Subject: Re: [Aroid-l] Sauromatum x Pinellia hybrid.... and others...
> From: James Waddick
> To:
Discussion of
aroids
aroid-l@www.gizmoworks.com
....
> In this same
discussion, the cv "Indian Giant' is brought to
> mind.
This is a much larger form of S. venosum. Could it be a
>
tetraploid? Has anyone compared chromosome numbers to the typical,
> smaller form? And is this, by the way, any more or less hardier
than
> the typical form?
Dear Jim W.,
Aroiders,
already more than one year
ago I compared the ploidy level of a normal form of Typhonium venosum (a
genotype/tuber that came from
Laos
) with my 'Indian
Giant' clone that was originally introduced by
Tony Avent
(Plant Delights Nursery).
Furthermore I have the clone that was given to me as T. nubicum. but is
probably identical with 'Indian Giant'.
For
pictures see: (scroll down to the post from 06.11.2009)
http://www.amorphophallus-forum.de/viewtopic.php?f=9&t=316&start=30
I did not count the
chromosomes but used flowcytometry to compare the DNA content.
In short words with this
method the DNA content of nuclei is measured after the DNA was
stained.
The measured values are
shown in a graph.
The first peak represents
the cells in the G1-phase; this would give the DNA-content of somatic
cells. The second peak represents the nucelar DNA content of cell in the
G2-phase after the replication of the DNA before the cell devides.
The graphs show the
measurements of T. venosum '
Laos
', T.venosum 'Indian
Giant' and T. nubicum, respectively.
[img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_v_Laos.jpg[/img]
[img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_v_IndianGiant.jpg[/img]
[img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_nubicum.jpg[/img]
The results
strongly indicate that the three clones do not differ in DNA content and
therefore have the same ploidy level.
However, I did no chomosome counts, but if 'Indian Giant'
would be a tetraploid of the normal form of T. venosum, the graph should
show a first peak at channel 100 for the G1-nuclei and a second at
channel 200 for the G2-nulei. This is not the case; all three clones
have the peaks roughly at channel 50 and channel 100.
Best, Bernhard.
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