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Re: Amorph TC
From: Michael Marcotrigiano <marcotrigiano at pssci.umass.edu> on 1998.06.03 at 18:34:38(2228)|
Toby: A good literature search will come up with several protocol. I know
of two decent references. One in Annals of Botany 57:11-17, the other in
Acta Horticulturae 226 (1988) page 65. Hope this helps.
At 10:49 PM 6/2/98 -0500, you wrote:|
> I was wondering if anyone has any experience in the microprop of
>Amorphophallus sp, and also if anyone has used either PPM or silver nitra=
>for bacteria inhibition/shoot stimulation in the TC of these. I don't wan=
>to steal anyone's 'Granny's secret recipe', but any indication of which
>auxins/cytokinins are generally effective and at what concs, what sort of=
>level of sucrose, agar would be very much appreciated. In addition, does
>anyone know what level of sterilisation is required for live tissue of
>these species- has anyone used tuber borings etc?
>Sorry for all the questions and I know this might not be quite the right
>place to ask them but any help would be invaluable for a poor student ;-)=
>My Kindest Regards and good growing /everyone/,
>Orleton Manor, Orleton, LUDLOW
>Salop, SY8 4HR, ENGLAND
Rm 211 French Hall
Dept. of Plant and Soil Sciences
Univ of Massachusetts
Amherst, MA 01003-4210
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