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  Re: Addition: Chlorine Bleach (and Peroxide)
From: Alektra at aol.com on 2001.12.16 at 22:08:44(7925)
Nothing I have so far read about sodium hypochlorite, on this list or
elsewhere, suggests that anyone has ever found it inappropriate to use for
plants. I am going to go into more detail on my reasons for bringing up the
question in the first place.

Again, I repeat: Chlorine compounds such as sodium hypochlorite, calcium
hypochlorite, and NaDCC are precisely what tissue culturists use to clean
leaf-cuts and seeds. In fact, sodium dichloroisocyanurate can be used mixed
into the medium, right into the substrate, to discourage microbial growth and
safeguard the plant tissue!

These people do not seem to find a raised final pH to be a problem. For the
professionals among them, often there's a lot of money riding on the
culturing. And naturally, people who culture tissue for a hobby would start
demanding other disinfectants if they didn't like the results they get. If I
were using one of these chlorine compounds on a rhizome or cutting or seed to
plant or culture, I would rinse it afterwards, anyway.

Maybe one of you out there who is culturing tissue at home could comment on
this, or could ask the people on the various home tissue culturing lists, or
at kitchenculturekit.com. I would think that those of you who are doing all
that home tissue culturing to build up the santa-leopoldina species would
know about this.

Cost and availability are serious issues, I think. It is virtually impossible
to get specific plant microbicides in small quantities at a reasonable cost.
Specific plant microbicides are hard to get, impossible to mail order, and
sold in expensive bottles or boxes that are enormous in relation to the size
of a single dose.

I get awfully skittish at the idea of, hypothetically, spending a work day
taking a train out to the country to a farm store so I can spend twenty bucks
on a liter of something just to get 5 or 10 ml, or a pound to get a fraction
of an ounce.

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